Studies of the UNC-45A Molecular Chaperone Expression in Human Cancer

dc.contributor.advisorBoehning, Darren F
dc.contributor.committeeMemberEpstein, Henry F
dc.contributor.committeeMemberBarral, José M
dc.contributor.committeeMemberMay, Gregory S
dc.contributor.committeeMemberTang, Shao-Jun
dc.creatorGuo, Wei
dc.date.accessioned2016-11-01T18:12:55Z
dc.date.available2016-11-01T18:12:55Z
dc.date.created2011-12
dc.date.submittedDecember 2011
dc.date.updated2016-11-01T18:12:55Z
dc.description.abstractThe UCS (UNC-45; Cro-1; She4p) domain proteins are highly evolutionarily conserved proteins, from fungi to mammals, which interact with several types of myosin, involving proper myosin functions. Caenorhabditis elegans UNC-45 acts as a molecular chaperone for myosin. Vertebrate genomes encode two UNC-45 genes: the UNC-45A(a) (general cell isoform) is expressed in many organs, whereas the UNC-45B(b) (striated muscle isoform) is exclusively expressed in striated muscle tissues. Our project reported UNC-45A mRNA and protein expression elevated in human cell lines derived from breast carcinoma metastases. UNC-45A knockdowns decreased breast cancer cells proliferation and invasion rates. We also indicated that UNC-45A(a) existed as two isoforms – UNC-45A(a) 929 and 944 – in mammalian cells. UNC-45A(a) 929 isoform had a long 5' untranslated region (UTR) and 944 isoform had a specific 15 amino acid sequence close to its N-terminus. Furthermore, we showed that UNC-45A 929 accumulated higher level in human breast cells. More importantly, the ubiquitin-proteasome system degraded endogenous UNC-45A and the 15 amino acid sequence unique to UNC-45A 944 protein mediated rapid turnover in mammalian cells.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2152.3/800
dc.subjectUNC-45
dc.subjectmyosin
dc.subjectbreast cancer
dc.subjectmolecular chaperone regulation
dc.subjectprotein degradation
dc.titleStudies of the UNC-45A Molecular Chaperone Expression in Human Cancer
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentCell Biology
thesis.degree.disciplineCell Biology
thesis.degree.grantorThe University of Texas Medical Branch at Galveston
thesis.degree.levelDoctoral
thesis.degree.nameCell Biology (Doctoral)

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Wei Guo Dissertation.pdf
Size:
2.09 MB
Format:
Adobe Portable Document Format

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.11 KB
Format:
Plain Text
Description: