2022-09-012022-09-012019-08August 201https://hdl.handle.net/2152.3/11729Intestinal Mesenchymal stromal cells (fibroblasts, myofibroblasts and their progenitors) have recently emerged as key players in the interplay between the microbiota and professional immune cells under homeostasis and chronic inflammation. Initiation of microbial interactions with host cells, including stromal cells, requires myeloid differentiation factor 88 (MyD88). Overall, MyD88-dependent interactions between the microbiota and the host is reported to contribute to epithelial barrier restoration via repositioning of mesenchymal stromal cells producing COX-2/PGE2 during injury. However, whether MyD88 signaling in mesenchymal cells is involved in this process is not known. Moreover, mesenchymal cell MyD88 signaling is involved in mucosal tolerance via suppression of type 1 adaptive immune responses. However, the impact of MyD88-dependent signaling within mesenchymal stromal cells on professional innate immune cells such as macrophages within the gut mucosa is poorly understood. Therefore, in this project I evaluate the hypothesis that MyD88-mediated signaling in mesenchymal stromal cells is required for the control over inflammatory responses in the colon. To test this hypothesis, I used primary human mesenchymal cells in culture, as well generated several mesenchymal stromal cell-specific MyD88-inducible knockout mice under homeostasis and in a model of acute colitis using DSS. Histological analysis was used to determine inflammatory and fibrotic changes in the colonic mucosa. The intracellular and cellular responses were analyzed by using RNAseq, qRT-PCR, ELISA, western blot, cytokine/chemokine multiplex array, flow cytometry, and confocal microscopy. In our first aim, I found that deletion of MyD88 within mesenchymal stromal cells in vivo resulted in inflammatory changes and increased fibrosis within the colonic mucosa as well as moderately aggravated DSS induced acute colitis. In addition to IBD-type dysbiotic changes of the fecal microbiota in mice lacking MyD88 within mesenchymal stromal cells, RNAseq analysis of the colonic mucosa from these mice revealed changes in pathways controlling epithelial barrier maintenance and innate and adaptive cell inflammatory responses in DSS-treated and non-treated animals. In order to understand how stromal cell-intrinsic MyD88 signaling activated by the microbiota contributes to the regulation of intestinal homeostasis and how it is disrupted during the immunopathogenesis of IBD, we used Lactobacillus rhamnosus GG (LbGG) as a representative species of normal beneficial gut microbiota. I demonstrated that mesenchymal stromal cells isolated from the normal human colonic mucosa responded to LbGG with activation of the eicosanoid pathway resulting in increased expression of COX-2 mediated PGE2. Similar observation were made in vivo, using a murine model. I also show that in culture and in vivo this process requires MyD88 signaling within mesenchymal stromal cells. Taken together, these data suggest that mesenchymal stromal cells are among the major contributors to the increase of COX-2-mediated PGE2 in response to the normal microbiota, contributing to the maintenance of mucosal homeostasis in the colon via MyD88. In experiments performed in my second aim, the inflammatory changes observed in our initial RNAseq analysis were associated with increased infiltration of F4/80+CD11b+ macrophages. Increase in CX3CR1highCCR2+ macrophages producing TNF-α was also observed and depletion of the macrophages with clondronate resulted in a decrease in total TNF-α levels within the colonic mucosa. This suggests that there is increased chemotaxis of inflammatory macrophages to the colonic mucosa. In conclusion, my data from my work suggest that MyD88 signaling within mesenchymal stromal cells contributes to colonic mucosal homeostasis through the production of PGE2 and the suppression of the influx of TNF-α and IL-6 producing inflammatory macrophages.application/pdf5-LO: 5-lypoxygenaseAA: Arachidonic AcidAJ: Adherens junctionsBM: Bone marrowCD: Crohn’s DiseaseCMFs: Colonic CD90+ (myo) fibroblastsCOX-2: Cyclooxygenase 2cPLA2: Cytosolic phospholipase A2DSS: Dextran Sulfate SodiumEB: Epithelial BarrierECM: Extracellular matrixER-TR7: anti-reticular fibroblasts and reticular fibres antibodyFib-MyD88 KO:Tamoxifen-inducible MyD88 floxed micespecific to Col1α2+ cellsGI: Gastrointestinali.p.: intraperitoneal injectionIEC: Intestinal Epithelial CellsIFN-γ: Interferon gammaIL-1: interleukin 1 receptorsIL-1: Interleukin-1IL-10: Interleukin-10IL-12: Interleukin-12IL-6: Interleukin-6ILC: Innate lymphoid cellsIPA: Ingenuity pathway analysisKO: KnockoutLbGG: Lactobacillus rhamnosus GGMC: Micro-colitisMF-MyD88 KO: Tamoxifen-inducible MyD88 floxed micespecific to α-SMA+ cellsMFs: Myofibroblasts and fibroblastsMLN: Mesenteric lymph nodeMRS: De Man, Rogosa and SharpeMSC: Mesenchymal stem cellsMyD88: Myeloid differentiation factor 88PDGFRα: platelet-derived growth factor receptor APD-L1: Programed death ligand 1PGE2: Prostaglandin E2Th: T-HelperTJ: Tight junctionsTLRs: Toll-like receptorsTMX: TamoxifenTNF- α: Tumor necrosis factor- αTreg: Regulatory T cellsUC: Ulcerative ColitisWB: Western blotα-SMA: smooth muscle actinThesis2022-09-01