Characterization of serum-induced CYP1A1 expression and activity in mouse embryo fibroblasts

dc.contributor.advisorCornelis Elferink Ph.D.en_US
dc.contributor.committeeMemberXiaodong Cheng Ph.D.en_US
dc.contributor.committeeMemberJonathan B. Ward Ph.D.en_US
dc.creatorCarmen-Veronica Naira Obianwuen_US
dc.date.accessioned2011-12-20T16:05:06Z
dc.date.available2008-06-17en_US
dc.date.available2011-12-20T16:05:06Z
dc.date.created2005-07-27en_US
dc.date.issued2005-07-07en_US
dc.description.abstractThe AhR is a ligand-activated transcription factor that mediates the toxic effects of environmental contaminants that include TCDD. Using a TCDD dose-response treatment in MEFs, we observed a super induction of CYP1A1 with newborn calf serum (NCS) in the presence (10nM/15nM) of TCDD. In addition to NCS, fetal bovine serum (FBS) also has the capability to yield a CYP1A1 super induction. These results suggest that components within the sera affect the activity of the AhR and consequent CYP1A1 expression. To pursue this idea, characterization of the serum factors were investigated. The findings indicated that serum factor(s) in both sera are heat sensitive at 50◦C, withstand removal from charcoal stripping sera and are ¡Ý 10,000 kDa in size. Using RT-PCR, we found that NCS factors only, could super induce CYP1A1 at the gene level. Moreover, MEFs are the only cells observed in this study that are susceptible to CYP1A1 super induction.en_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-07272005-110732en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/192
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectTCDDen_US
dc.subjectserumen_US
dc.subjectCYP1A1en_US
dc.subjectAHRen_US
dc.titleCharacterization of serum-induced CYP1A1 expression and activity in mouse embryo fibroblastsen_US
dc.type.genrethesisen_US
dc.type.materialtexten_US
thesis.degree.departmentPharmacology and Toxicologyen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelMasteren_US
thesis.degree.nameMaster of Scienceen_US

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