Differences in miR-122 and miR-191 Expression in HBV- versus HCV-associated Hepatocellular Carcinoma
| dc.contributor.advisor | Lemon, Stanley M | |
| dc.contributor.committeeMember | Bachenheimer, Steven L | |
| dc.contributor.committeeMember | Yi, MinKyung | |
| dc.creator | Spaniel, Carolyn | |
| dc.date.accessioned | 2016-11-14T15:24:47Z | |
| dc.date.available | 2016-11-14T15:24:47Z | |
| dc.date.created | 2012-12 | |
| dc.date.submitted | December 2012 | |
| dc.date.updated | 2016-11-14T15:24:48Z | |
| dc.description.abstract | Prior studies have suggested that the expression of miR-122 and miR-191 is decreased and increased, respectively, in hepatocellular carcinoma. However, other evidence suggests miR-122 may be preserved in HCV-associated hepatocellular carcinoma (HCC). The goal of this thesis is to assess miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC, the related non-tumor tissue, and normal liver tissue. Relative quantification of miR-122 and miR-191 in 16 HCV-associated HCC, 10 HBV-associated HCC, the respective neighboring non-tumor tissue, and 9 normal liver tissue samples was performed using RT-PCR. This thesis shows that miR-122 expression levels are maintained in HCV-associated HCC and HBV-non-tumor tissues, but down-regulated in HBV-associated HCC and HCV-non-tumor tissues compared to normal liver tissue. miR-191 was found only to be up-regulated in HBV-associated HCC compared to normal liver expression levels. Furthermore, the miR-122 expression level in HCV-non-tumor tissue was found to have a relationship with rs8099917, a SNP known to predict HCV treatment outcome. miR-122 expression levels were decreased in patients with the TG genotype, unfavorable to HCV treatment, compared to those with the TT genotype, favorable to HCV treatment. A negative correlation of interferon-stimulated gene expression and miR-122 expression was found using Spearman correlation tests. The differences in miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC hint that there are virus-specific mechanisms that influence liver carcinogenesis during chronic infection. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/2152.3/850 | |
| dc.subject | miR-122, miR-191, hepatocellular carcinoma, interferon, liver cancer | |
| dc.title | Differences in miR-122 and miR-191 Expression in HBV- versus HCV-associated Hepatocellular Carcinoma | |
| dc.type | Thesis | |
| dc.type.material | text | |
| thesis.degree.department | Microbiology and Immunology | |
| thesis.degree.discipline | Virology | |
| thesis.degree.grantor | The University of Texas Medical Branch at Galveston | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Microbiology and Immunology (Masters) |