THE ROLE OF INTEGRATOR SUBUNIT 11 IN PROMOTOR-PROXIMAL ATTENUATION OF PROTEIN CODING GENES
| dc.creator | Elrod, Nathan David | |
| dc.creator.orcid | 0000-0003-1310-6026 | |
| dc.date.accessioned | 2022-01-18T16:29:00Z | |
| dc.date.available | 2022-01-18T16:29:00Z | |
| dc.date.created | 2021-12 | |
| dc.date.submitted | December 2021 | |
| dc.date.updated | 2022-01-18T16:29:02Z | |
| dc.description.abstract | The Integrator complex is a 14-subunit protein complex that interacts with RNA polymerase II (RNAPII) in metazoans during transcription of multiple forms of coding and non-coding RNA. Originally described as being responsible for the 3’ cleavage and termination of uridine-rich small nuclear RNA, further roles have been discovered in the cleavage of long non-coding RNA, enhancer RNA, telomerase RNA, and coding messenger RNAs. While many studies have identified a presence at the transcriptional start site of mRNA, Integrator’s function at these mRNA has yet to be identified. In this study, we used multiple biochemical and sequencing techniques to elucidate a role for Integrator at these mRNA start sites. We found that the endonuclease activity of Integrator subunit 11 (IntS11) was responsible for the attenuation of mRNA through promoter-proximal termination of a large set of paused mRNAs. We also identified a novel interaction between IntS11 and CG7044 that could inhibit IntS11’s endonuclease activity. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://hdl.handle.net/2152.3/11363 | |
| dc.subject | RNA biology, Transcription | |
| dc.title | THE ROLE OF INTEGRATOR SUBUNIT 11 IN PROMOTOR-PROXIMAL ATTENUATION OF PROTEIN CODING GENES | |
| dc.type | Thesis | |
| dc.type.material | text | |
| thesis.degree.department | Biochemistry and Molecular Biology | |
| thesis.degree.grantor | The University of Texas Medical Branch at Galveston | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Biochemistry and Molecular Biology (Doctoral) |