Evaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M. bovis DeltaRD1

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dc.contributor.advisorD. Mark Estesen_US
dc.contributor.committeeMemberSlobodan Paessleren_US
dc.contributor.committeeMemberJanice J. Endsleyen_US
dc.contributor.committeeMemberGregg N. Milliganen_US
dc.contributor.committeeMemberBernardo Villarreal-Ramosen_US
dc.contributor.committeeMemberAlfredo G. Torresen_US
dc.creatorCharles Fernando Capinos Schereren_US
dc.date.accessioned2011-12-20T16:05:46Z
dc.date.available2010-09-28en_US
dc.date.available2011-12-20T16:05:46Z
dc.date.created2008-12-31en_US
dc.date.issued2008-09-18en_US
dc.description.abstractThe development of improved vaccines against tuberculosis (TB) is directly linked to the investigation of new and better correlates of protection after vaccination against TB. Cloning and characterization of bovine homologues of the antimicrobial protein granulysin (Bo-lysin) and perforin by our group could be used as potential biomarkers for TB vaccination efficacy. In the present study we examined the kinetics of granulysin, perforin, IFNgamma and Fas-L responses to Mycobacterium bovis purified protein derivative (PPD) stimulation by peripheral blood mononuclear cells from M. bovis DeltaRD1-, BCG- and non-vaccinated cattle. Gene expression profiles following PPD stimulation showed significant increases in transcripts for granulysin and IFNgamma in both CD4+ and CD8+ T cells in BCG-vaccinated as compared to non-vaccinated animals. Perforin and IFNgamma examined by flow cytometry, showed a difference of 1-2% more PPD-specific cells in BCG-vaccinated than non-vaccinated animals. In the vaccine trial, granulysin and perforin were significantly increased in both vaccine groups as compared to control after vaccination and challenge. IFNgamma expression was increased only after vaccination and secretion was higher in the control, as compared to both vaccine groups demonstrating no correlation with protection upon vaccination. In summary, results shown here provide evidence that granulysin and perforin are prospective candidates as biomarkers of protection after vaccination against TB.en_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-12312008-061540en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/300
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectvaccinationen_US
dc.subjectperforinen_US
dc.subjectMycobacterium bovisen_US
dc.subjectgranulysinen_US
dc.subjectbiomarkersen_US
dc.titleEvaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M. bovis DeltaRD1en_US
dc.type.genredissertationen_US
dc.type.materialtexten_US
thesis.degree.departmentMicrobiology and Immunologyen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhDen_US

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