Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal

Simple item page
dc.contributor.advisorKathryn A. Cunninghamen_US
dc.contributor.committeeMemberXiaodong Chengen_US
dc.contributor.committeeMemberNorbert K. Herzogen_US
dc.contributor.committeeMemberMary L. Thomasen_US
dc.creatorTao Gengen_US
dc.date.accessioned2011-12-20T16:05:08Z
dc.date.available2008-06-17en_US
dc.date.available2011-12-20T16:05:08Z
dc.date.created2005-07-29en_US
dc.date.issued2005-07-22en_US
dc.description.abstractSELDI-TOF MS is an analytical technology that combines chromatography and mass spectrometry. It is being exploited for the measurement of numerous proteins in serum, less often in organ tissue (rarely in brain). We applied SELDI-TOF MS technology to study protein expression in the brain of rats withdrawn from repeated cocaine exposure. Our goals were to optimize sample preparation and ProteinChip„¥ Array protocols for brain tissue, to verify the reproducibility of SELDI-TOF mass spectra, and to determine whether SELDI-TOF MS detects differentially expressed proteins in cocaine- vs. saline-treated rats. Consequently, we have developed an optimal protocol and generated a reproducible spectra with six dominant peaks in all test samples. We have found two peaks (m/z: 5179, 5030) significantly increased (p <. 05) in cocaine-treated rats. In summary, the application of SELDI-TOF MS to the study of protein expression in a rat model of cocaine withdrawal is feasible and potentially informative.en_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-07292005-113525en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/198
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectproteomics brainen_US
dc.titleUse of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawalen_US
dc.type.genrethesisen_US
dc.type.materialtexten_US
thesis.degree.departmentPharmacology and Toxicologyen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelMasteren_US
thesis.degree.nameMaster of Scienceen_US

Files

Original bundle

Now showing 1 - 3 of 3
No Thumbnail Available
Name:
Handbook-front.doc
Size:
81.5 KB
Format:
Microsoft Word
Download
No Thumbnail Available
Name:
Handbook2005-sep.doc
Size:
726 KB
Format:
Microsoft Word
Download
No Thumbnail Available
Name:
VITA.doc
Size:
39 KB
Format:
Microsoft Word
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
891 B
Format:
Plain Text
Description:
Download

Collections

Published ETD Collection