PTEN loss induces epithelial-mesenchymal transition in human colon cancer cells

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dc.contributor.advisorB. Mark Eversen_US
dc.contributor.committeeMemberTien Koen_US
dc.contributor.committeeMemberSarita Sastryen_US
dc.contributor.committeeMemberPeter Zhouen_US
dc.contributor.committeeMemberDai Chungen_US
dc.creatorKanika Alake Bowenen_US
dc.date.accessioned2011-12-20T16:04:43Z
dc.date.available2010-09-28en_US
dc.date.available2011-12-20T16:04:43Z
dc.date.created2009-06-17en_US
dc.date.issued2009-06-16en_US
dc.description.abstractBackground: Epithelial-mesenchymal transition is a critical early event in the invasion and metastasis of many cancers, including colorectal cancer. Chronic inflammation is an inducer of several cancers and inflammatory cytokines have been implicated in tumor invasion. Materials and Methods: Human colon cancer cell lines HCT116 and SW480 were transfected with PTEN siRNA or non-targeting control. Invasiveness was measured using a modified Boyden chamber assay and migration was assessed using a scratch assay. Results. PTEN knockdown increases the invasion and migration of CRC cells and the addition of medium containing TNF-á further enhanced the migration and invasion. PTEN knockdown resulted in nuclear â-catenin accumulation and increased expression of downstream proteins c-Myc and cyclin D1. Conclusions. Our study supports clinical studies identifying an association of PTEN loss with late stage cancer. Cellular factors secreted from the surrounding tumor milieu likely act in concert with genetic changes in the tumor cells and contribute to enhanced tumor invasion.en_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-06172009-115515en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/121
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectPTENen_US
dc.subjectepithelial-mesenchymal transitionen_US
dc.subjectcolon canceren_US
dc.titlePTEN loss induces epithelial-mesenchymal transition in human colon cancer cellsen_US
dc.type.genremajor_paperen_US
dc.type.materialtexten_US
thesis.degree.departmentCell Biologyen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelMasteren_US
thesis.degree.nameMaster of Medical Scienceen_US

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