The construction and characterization of recombinant sindbis virus vectors as a platform for anthrax vaccine development

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dc.contributor.advisorJohnny W. Petersonen_US
dc.contributor.committeeMemberThomas Wooden_US
dc.contributor.committeeMemberRolf Konigen_US
dc.contributor.committeeMemberHans Heidneren_US
dc.creatorThomas, Johnen_US
dc.date.accessioned2011-12-20T16:05:39Z
dc.date.available2008-12-10en_US
dc.date.available2011-12-20T16:05:39Z
dc.date.created2008-12-01en_US
dc.date.issued2008-11-13en_US
dc.description.abstractThe intentional release of anthrax spores in the U. S. mail system and the subsequent fatalities have renewed interest in developing therapeutics to protect against B. anthracis. Anthrax Vaccine Adsorbed (AVA) is the only licensed vaccine preparation currently used however, immunogenicity is variable and the boosting regimen is long-term and must be maintained to ensure continued protection. Protective antigen (PA), the chief component of AVA, is an 83kDa protein responsible for binding host cell receptors and promoting toxin entry into susceptible cells. Analysis of the PA molecule has identified a specific region, termed domain four, that contains epitopes responsible for antibody binding. Sindbis virus, the prototypical member of the genus alphavirus, has been extensively used as a vector of foreign gene expression. Several characteristics, including ease of manipulation, high levels of transgenic expression, and lack of pathogenicity in humans make Sindbis virus an attractive candidate as a vaccine vector. The purpose of this proposal is to construct and characterize replication-competent Sindbis virus vaccine vectors that express PA using in vivo and in vitro analysis in order to generate a recombinant viral vaccine capable of providing protection from virulent anthrax spore challenge.en_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-12012008-120339en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/278
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectt cellen_US
dc.subjectsporeen_US
dc.subjectSindbisen_US
dc.subjectdendritic cellen_US
dc.subjectanthraxen_US
dc.subjectAmesen_US
dc.titleThe construction and characterization of recombinant sindbis virus vectors as a platform for anthrax vaccine developmenten_US
dc.type.genredissertationen_US
dc.type.materialtexten_US
thesis.degree.departmentMicrobiology and Immunologyen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhDen_US

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