Reactive Oxygen Species and Lipid Peroxidation Products Contribute to Neuropathic Pain in Chronic Spinal Cord Injured Rats

Simple item page
dc.contributor.advisorHulsebosch, Claire E
dc.contributor.committeeMemberChung, Jin-Mo
dc.contributor.committeeMemberCarlton, Susan M
dc.contributor.committeeMemberKaphalia, Bhupendra S
dc.contributor.committeeMemberHall, Edward D
dc.creatorHassler, Shayne N
dc.date.accessioned2016-05-05T21:54:38Z
dc.date.available2016-05-05T21:54:38Z
dc.date.created2014-12
dc.date.submittedDecember 2014
dc.date.updated2016-05-05T21:54:38Z
dc.description.abstractSpinal cord injury (SCI) can result in loss of locomotion, sexual and bladder function, and chronic neuropathic pain. A rat model of spinal cord injury was used to study the mechanisms of chronic neuropathic pain. Chronically up-regulated reactive oxygen species (ROS) generated in neurons as a result of spinal cord injury contribute to the chronic neuropathic pain measured in rats. When ROS was reduced in chronic SCI by using PBN, a ROS scavenger, there was a reduction in neuropathic pain behaviors and a reduction in hyperexcitability of dorsal horn neurons in rats. Compounds that reduce ROS and lipid peroxidation are able to reduce measures of chronic neuropathic pain. Mechanical allodynia was reduced in chronic SCI rats with the intrathecal administration of Apocynin, 4-OXO-TEMPO, Tirilazad, and U-83836E. Apocynin was used to further examine the ability of ROS and lipid peroxidation products to generate neuropathic pain behaviors in chronic SCI rats. Apocynin was found to reduce expression of 4-HNE, a lipid peroxidation product, in chronic SCI rats when administered intraperitoneally 30 minutes before perfusion and fixation. Evoked and non-evoked measures of neuropathic pain were reduced in chronic SCI rats when Apocynin was applied intrathecally and intraperitoneally. Apocynin was also able to reduce hyperexcitability of dorsal horn neurons in chronic SCI rats. These findings indicate that ROS is a major contributor to chronic neuropathic pain resulting from SCI. Further investigation of the relationship between reactive oxygen species and chronic neuropathic pain may lead to better understanding of the biological processes of chronic pain and more effective therapies for treating injury induced neuropathic pain.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2152.3/727
dc.subjectChronic Pain, Neuropathic Pain, Spinal Cord Injury, Allodynia, Reactive Oxygen Species, Lipid Peroxidation
dc.titleReactive Oxygen Species and Lipid Peroxidation Products Contribute to Neuropathic Pain in Chronic Spinal Cord Injured Rats
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentNeuroscience
thesis.degree.disciplineNeuroscience
thesis.degree.grantorThe University of Texas Medical Branch at Galveston
thesis.degree.levelDoctoral
thesis.degree.nameNeuroscience (Doctoral)

Files

Original bundle

Now showing 1 - 2 of 2
Thumbnail Image
Name:
HASSLER-DISSERTATIONDOCTORAL-2014.pdf
Size:
2.31 MB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
Hassler Dissertation.doc
Size:
8.45 MB
Format:
Microsoft Word
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
1.84 KB
Format:
Plain Text
Description:
Download

Collections

Published ETD Collection